Return Home


Order Form
Policies
Contact Info



Preliminary clinical studies continue to show the promise of ultra-hydrolyzed proteins as a trigger for increased insulin response and greatly enhanced delivery of key aminos to nutrient-starved muscle tissue. In addition, supplementation with leucine has been linked to endurance and upper-body power, as well as protein synthesis. The below clinical abstract summaries serve to illustrate the results of these key studies for your reference and review.

  • An Open Label Clinical Trial Evaluating the Effects of MyoZene™ with Resistance Training on Changes in Body Composition and Muscle Strength.

    Author Block: Lidia Lou, Douglas Kalman, Samantha Feldman, Diane R. Krieger. Miami Research Associates, Miami, FL. (Sponsor: Jose Antonio PhD, FACSM)
    Email: llou@miamiresearch.com

    Abstract:
    PURPOSE: This study explored the efficacy of post workout supplementation with the sports nutrition product MyoZene, a protein-based product with creatine, on enhancing lean body mass and promoting strength gains when used in combination with a standardized resistance training program. METHODS: Twelve active healthy males, at least 18 years of age, with a minimum of 6 month history of resistance weight training were enrolled into a 4-week open label study of daily supplementation of a protein creatine product in combination with a 6-day split body resistance training program. Body composition was assessed via DXA and strength/endurance via performance of bench press and leg press exercises to exhaustion. Three warm up sets were completed prior to 2 sets of maximum repetitions to failure (sets 4 and 5), using 100% body weight for bench press and 200% body weight for leg press. RESULTS: Subjects tended to gain fat free mass (+0.97±1.55 kg; p=0.053) while slightly reducing the percent of body fat (-0.63±1.20%; p=0.095). Significant increases were seen in upper body strength/endurance [increased max reps for sets 4 and 5 at 4 weeks (+2.58±2.84 and +3.0±2.09; p=0.009 and <0.001 respectively)] as well as lower body strength/endurance [increased max reps for sets 4 and 5 at 4 weeks (+5.4±4.6 and +8.6±10.2; p= 0.002 and 0.004 respectively)]. The increases in strength from baseline were 24% mid-study and 32% at study end. There was no safety concerns noted. CONCLUSION: This is the first study with a protein-creatine product to show increases in muscular endurance/strength as high as 32%. Previous studies have found increases in the range of 5 to 15%. Additional research with a control group and longer duration is warranted.

    1. An Open Label Clinical Trial Evaluating the Effects of MyoZene with Resistance Training on Changes in Body Muscle Strength." Lidia Lou, MS, Samantha Feldman MS, RD, William Chong BS, Douglas Kalman PhD, RD, FACN, Diane R. Krieger, MD. May 2008. Presented at the 2008 American College of Sports Medicine Annual Meeting in Indianapolis, IN.


  • Clinical Research Supports the Mass-Building Superiority of Whey Protein Hydrolysates
    The groundswell of clinical and anecdotal evidence supporting the superior anabolic efficacy of ultra-hydrolyzed proteins continues to gather momentum. The most recent scientific study, published in the International Journal of Sports Nutrition and Exercise Metabolism, examined the effects of supplementation with different proteins, namely hydrolyzed whey protein and casein (the main component of milk protein), on muscle strength and body composition during a 10 week, supervised resistance training program.1 Importantly, this study was conducted on experienced male bodybuilders. In a double-blind protocol, these subjects supplemented their normal diet with either whey hydrolysate or casein (1.5 grams per kilogram of body mass/day). What happened? Well, this well-controlled study indicated that :

      1. The whey hydrolysate group achieved a significantly greater gain in lean body mass than the casein group (5.0 vs. 0.8 kilograms). Also, the whey hydrolysate group lost significantly more body fat while the casein group gained body fat (-1.5 vs. +0.2 kilograms).

      2. The whey hydrolysate group also achieved significantly greater improvements in muscle strength (measured by barbell bench press, squat and cable pull-down) compared to the casein group in each assessment of strength. Furthermore, when the strength changes were expressed relative to body weight, the whey group still achieved significantly greater improvements in strength compared to the casein group.
    In conclusion, the superiority of whey protein hydrolysate may have something to do with its insulin boosting actions and its extremely rapid absorption and uptake.2 Interestingly enough, this study also demonstrated whey hydrolysate ingestion promoted fat loss; high-quality whey has ACE-inhibitory activity, which leads to inhibition of fat storage.


  • Ultra-hydrolyzed proteins drive faster increases in insulin secretion and amino acid availability to muscle tissue
    A recent study at the Copenhagen Muscle Research Center was implemented to determine the effects different protein-containing solutions have on insulin response and amino acid availability in healthy humans. Four different solutions of 600 milliliters were used in this study. The glucose solution (control) contained only glucose, and the three additional solutions contained the same quantity of glucose and protein, but proteins were derived from different sources. This study indicated that:

      1. Ingestion of glucose and protein hydrolysate results in synergistic and fast increases in blood insulin. In fact, protein hydrolysates stimulated an increase in blood insulin that was two and four times greater than that produced by the intact (non-hydrolyzed) milk protein solution and glucose solution, respectively.

      2. Protein hydrolysates are absorbed at a faster rate from the small intestine than are intact milk proteins, as reflected by the rapid increase in the blood concentration of branched-chain amino acids (BCAAs) in peripheral blood.

      3. Whey protein hydrolysate elicited the greatest availability of amino acids during the three-hour postprandial (occurring after a meal) period. This difference was attributed to the rapid increase in blood amino acids evoked during the first 40 minutes of the digestive period, during which the increase was about 37% greater after the ingestion of whey protein hydrolysate solution than that after ingestion of the intact milk protein solution. The authors suggested that the association of high levels of blood amino acids and insulin might explain a superiority of protein hydrolysates over intact proteins in promoting better nitrogen utilization (i.e., greater anabolism), especially when administered in combination with high glycemic carbohydrates.

    Calbet JA, MacLean DA. Plasma glucagons and insulin responses depend on the rate of appearance of amino acids after ingestion of different protein solutions in humans. J. Nutr. 2002 Aug;132(8):2174-82.


  • Protein hydrolysate increases insulin levels during post-exercise recovery
    Dr. Kaastra and co-workers determined the extent to which the combined ingestion of high-glycemic carbs and a protein hydrolysate (rich in small peptides), with or without additional free leucine, can increase insulin levels during post-exercise recovery.

    Fourteen male athletes were subjected to three randomized crossover trials in which they performed 2 h of exercise. Thereafter, subjects were studied for 3.5 h during which they ingested carbs only, carbs + protein hydrolysate, or carbs + protein hydrolysate + free leucine in a double-blind fashion. The results revealed that blood insulin responses were 108% and 190% greater in the carbs + protein hydrolysate and carbs + protein hydrolysate + leucine trial, respectively, compared with 24 25 the carbs only trial. This study also indicates that addition of free phenylalanine, as applied in earlier studies, is not necessary to obtain such high post-exercise insulin responses.
    Kasatra B, et al. Effects of increasing insulin secretion on acute post-exercise blood glucose disposal. Med Sci Sports Exerc. 2006 Feb;38(2):268-75.


  • Up to 221% increases in blood insulin response
    A study at the Maastricht University examined blood insulin responses after co-ingestion of protein hydrolysate (rich in small peptides) with and without additional free leucine with a single bolus of high-glycemic carbohydrate. Subjects participated in 3 trials in which blood insulin responses were determined after the ingestion of beverages of different composition: carbs only, carbs + protein hydrolysate, or carbs + protein hydrolysate + free leucine.

    The result indicated that blood insulin responses were 66% and 221% greater in the healthy controls in the carbs + protein hydrolysate and carbs + protein hydrolysate + free leucine trials, respectively, compared with those in the carbs only trial. In other words, this study also demonstrated that co-ingestion of a protein hydrolysate with additional leucine strongly augments insulin secretion after the consumption of a single bolus of carbohydrate.
    Manders RJ, et al. Co-ingestion of a protein hydrolysate with or without additional leucine effectively reduces post-prandial blood glucose excursions in type 2 diabetic men. J. Nutr. 2006 May;136(5): 1294-9.


  • Protein/free leucine combination increases protein synthesis
    A well-controlled study by Dr. Koopman and colleagues examined post-exercise muscle protein synthesis and whole body protein balance following the combined ingestion of highglycemic carbohydrate with or without whey protein hydrolysate and/or leucine. The results revealed that the co-ingestion of leucine even further increases the net protein balance, compared with carbohydrate and whey protein hydrolysate ingestion. The authors concluded that, "the additional ingestion of free leucine in combination with protein and carbohydrate likely represents an effective strategy to increase muscle anabolism following resistance exercise."
    Koopman R, et al. Combined ingestion of protein and free leucine with carbohydrate increases post-exercise muscle protein synthesis in vivo in male subjects. Am J Physiol Endocrinol Metab. 2005 Apr;288(4):E645-53.


  • Leucine supplementation improves endurance and upper body power
    Dr. Crowe and co-workers investigated the effects of leucine supplementation on the exercise performance of well-trained athletes. The subjects underwent testing before and after six weeks of supplementation with either leucine or placebo. The results revealed that leucine supplementation significantly improved endurance performance and upper body power. Researchers suggested that the performance-enhancing effects of leucine were likely related to a reduction in skeletal muscle damage with training and/or an increase in skeletal muscle protein anabolism.
    Crowe MJ et al. Effects of dietary leucine supplementation on exercise performance. Eur J Appl Physiol. 2005 Oct 29: 1-9.

Terms of Use | Disclaimers
© 2010 BioQuest, Inc. A Division of NutraQuest, Inc. All Rights Reserved.

Healthy weight management and substantial weight loss are best achieved by making lifestyle changes, such as lowering caloric intake and increasing exercise activity. Clinical evidence indicates that when used in combination with a sensible diet and exercise, the key ingredients can support your weight loss program. Consult a health care professional before beginning any weight-loss program. Read and follow all label instructions before using. Endorsers used this product in conjunction with diet and exercise and were remunerated for their appearance. These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.